|
KMID : 1130320100530070753
|
|
Korean Journal of Pediatrics
2010 Volume.53 No. 7 p.753 ~ p.758
|
|
|
Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line
|
|
Kim Sun-Young
Lee Kyung-Yil
Jeong Chang-Mo
Kim Hack-Ki
|
|
|
Abstract
|
|
|
Purpose: It has been suggested that p16 has a role in glucocorticoid (GC)-related apoptosis in leukemic cells, but the exact mechanisms have yet to be clarified. We evaluated the relationship between the GC response and p16 expression in a lymphoma cell line.
Methods: We used p16 siRNA transfection to construct p16-inactivated cells by using the B-cell lymphoblast cell line NC-37. We compared glucocorticoid receptor (GR) expression, apoptosis, and cell viability between control (p16+ NC-37) and p16 siRNA-transfected (p16? NC- 37) cells after a single dose of dexamethasone (DX).
Results: In both groups, there was a significant increase in cytoplasmic GR expression, which tended to be higher for p16+ NC-37 cells than for p16? NC37 cells at all times, and the difference at 18 h was significant (P<0.05). Similar patterns of early apoptosis were observed in both groups, and late apoptosis occurred at higher levels at 18 h when the GR had already been downregulated (P<0.05). Cell viability decreased in both groups but the degree of reduction was more severe in p16+ NC-37 cells after 18 h (P<0.05).
Conclusion: These results suggest a relationship between GR expression and cell cycle inhibition, in which the absence of p16 leads to reduced cell sensitivity to DX.
|
|
|
KEYWORD
|
|
|
Glucocorticoid, Lymphoblast, p16
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|